Understanding The Complexities Of Life Education Essay

My schooling and college was real of spell by factor in determining my calling. tenuous classs in board scrutinies, 83 % in tenth class and 82 % in 12th class, always kept me in top 5 % of the school. slight mental faculty, which ever foc exampled on the basicss, motivated me to meditate harder and with complete apprehension of the topic. In my college, I became strangely fire in the country of Biology as I was ever amazed by the sort and complexness of support around me I chose to conk bring emerge biological cognition in more(prenominal) item. I chose Biotechnology as my major for my bachelorsA grade ( B.Sc. ) along with chemical perception and fauna as furnish leagues. Biotechnology helped me infrastand apply biological comprehension in great inside informations. I also attended an on occupation bourgeoning under Annual Biotechnology Training plan at Haffkin land, Mumbai where I got more exposure to practical biotechnology, diverse biophysical proficie ncys and instrumentality. match to all this, I was bothway interested in figuring molds right from my school yearss. A class on programming in C, gave me an prognosis to produce computing machine scheduling and its exercises. Having hear of bioinformatics as a combination of biological information and computing machines, I applied for and got selected through plumping construe of applications to go to a store on bioinformatics at Indian Institute of Technology ( IIT ) , Mumbai. Here, I was introduced to power of computing machines to reply inquiries in biological acquisition. My apprehension of biological apprehension, love towards computing machines and sharpness into the universe of bioinformatics and its applications through my course of study and the take inshop buy the farmed as a strong motive for me to compress the specialisation for my master key s grade. I unconquerable to prosecute my farther surveies in the field of bioinformatics and computational bi ological perception with an purpose of utilizing potencies of computing machine systems and of young promotions in the field of information design in sound out to analyze biological science with more easiness and at accelerated rate.I was selected to prosecute my Maestro of Science ( MSc ) in bioinformatics at Bioinformatics Centre, University of Pune, India, through a realmwide debut scrutiny. I was ranked 8th from around green appliers who appe atomic number 18d for the trial. My systematically good public inaugurateation in entryway trial and in subsequent semesters do me eligible for Prof G. N. Ramchandran family and Dept. of Biotechnology, Govt. of India scholarship to prosecute my professional s point in time instruction. Bioinformatics Centre is one of the primal Minister institutes in India which is renowned for its part in the bioinformatics seek and in developing good bioinformatics piece resource. Two old ages of strict preparation in bioinformatics taught me a bay window just close to this astonishing spinal fusion of biological scientific disciplines and information scientific disciplines. I was introduced to disparate computing machine scheduling linguistic communications and resources for application using in bioinformatics. Classs in biological science, computing machines and accent on their earmark incorporate made me believe of biological science in a sincerely different and outlaw(a) mode. In our 2nd semester, we had a qualified geomorphologic Biology and Molecular Modeling ( SBMM ) , where we were introduced to bio-macromolecular(a)(a) grammatical sayings with a computational and biophysical position. With the survey for this peculiar class, I became more focussed on my country of employment. Surveies of geomorphologic biological science of proteins were truly an evoke portion of my course of study. uplifted scientists from different national research institutes gave us penetrations into the morphological biol ogical science of different biomolecules. I nooky non motivity without adverting names of some of my instructors, Prof Ashok Kolaskar ( adviser, OHSL, the States and adviser, Internet2 ) , Dr. Dhananjay Bhattacharyya ( Saha Institute of Nuclear Physics ) , Dr. Raja Banerjee ( western Bengal University of Technology ) , Dr. Udd assumesh Sonawane ( C-DAC ) , Prof P. V. Balaji ( IIT, Mumbai ) to call a few.My introductory research receive in the field of Bioinformatics was my maestro s degree research pioneer under the counseling Dr. Pramod Wangikar in his Bio-systems Engineering Lab at Chemical Engineering section of Indian Institute of Technology ( IIT ) , Mumbai. Though it was non straight connected to my country of involvement, it gave me insights into how research methodological analysis bats for computational biological science. Here I put to worked with analysis of regulatory elements of cyanobacteria- Synechocystis as a little portion of defective systems biological science undertaking traveling on in his lab. This undertaking, as a whole, was taking towards constructing a systemic notional account of Synechocystis. My part was to use microarray informations for cistron look of all Synechocystis cistrons and written textual matter factor adhering site analysis in place to footnote some of the cistrons. I could foretell the constitute of two cistrons which found to be re slowd to photosynthetic tract but were unknown boulder cadaver day of the month. I got familiar with the existent life applications of different tools and techniques that I had studied earlier. From here, I determine to prosecute my calling in the research field and apply as per my capablenesss to the scientific community.After MSc, I worked under the counsel of Prof Valadi Jayaraman from Center for suppuration of Advanced Computing ( C-DAC ) , India. I worked with Prof Jayaraman on short term undertaking titled prescience of ribonucleic acid adhering proteins utiliz ing random woods. His first-class counsel and my unvoiced work contributed to my apprehension of machine acquisition applications in biological science and utilizing information from biological twistings for break-dance apprehension of the biological processs. The undertaking involved filiation of of import characteristics from the RNA adhering sphere of many an(prenominal) RNA bond proteins and developing the random forest classifier to do accurate anticipations about RNA adhering belongingss of unknown proteins. This at a time more provided me with a really utile technique of machine acquisition for work outing jobs in biological science and also enhanced my completements of computing machine programming for existent life applications.Parallel to this work with Prof Jayaraman, I also worked in an industry, Persistent Systems Limited, Pune as Domain Analyst, where I learnt a batch about industrial position of bioinformatics and recent IT advancements assisting bioinformat ics grow. It gave me an chance to form my ideas, represent myself professionally and to larn clip and resource direction. I worked on supply preparation and support to many of US universities, our clients, for caTissue clinical information sciences application. But my passion and finding for fashioning research alert my head to go fore industry and carry out research.I applied for the station of Research Associate at Bioinformatics Centre, University of Pune. I got selected after strict interview procedure by an elegant decorate under the Centre of morality ( CoE ) grant of Dept. of Biotechnology ( DBT ) , Govt. of India. Here I started work on some of the authorities funded undertakings. I worked on Understanding the mechanism of anisomycin induced activation of p38 use kinase utilizing computational attack under the high counsel of Dr. Sangeeta Sawant. Anisomycin is an antibiotic, which in addition activates p38 MAP kinase, an of import constituent of presage transduct ion. But no information is available on how precisely it binds to and activates p38 MAP kinase. I attempted to ladder molecular docking surveies and molecular kineticss simulation experiments. This work resulted in happening out putative binding site for anisomycin on p38 MAPK and in seeing different molecular interactions taking topographic point at the interface of these two molecules. I learnt a batch about structural belongingss of proteins and power of MD Simulations to research mechanisms underlying cellular procedures. I used yellow-brown molecular kineticss simulation bundle extensively to hug medicate out the simulations. Another undertaking was Analyzing the comparative stablenesss of conformational antigenic determinants a instance survey of muramidase , which was carried out under the counsel of Dr. Sangeeta Sawant and Dr. Urmila Kulkarni-Kale. This survey was carried out to understand the comparative stablenesss of single antigenic determinants and happening out a ntigenic determinants which might retain their stableness during their processing. We could natural covering up the hypothesis that such antigenic determinants do be and can be identified. Possible application of this survey would be in placing possible drug/vaccine marks. We used blossoming simulation technique to analyze the phenomenon. I am happy to nation that a portion of this work was presented with batch of taste at International Conference on Biomolecular Forms and Functions, held at Indian Institute of Science, Bangalore and complete work is in concluding phases of its entry for publication. Both these undertakings equipped me with a posited attitude to transport out research and believe in a logical and keen-witted manner to come to a solution. It made my foundations about structural biological science, biophysical chemical science and computing machine simulations stronger than they were. ongoingly I am retention the same place and seeking to take my work to a logic al terminal.Presently, I am besides involved in the teaching of Chemical answer Optimization ( CRO ) establish cistron superior algorithm under the counsel of Prof Valadi Jayaraman of C-DAC, Pune. This undertaking work, which had merely started, is using my scheduling and bioinformatics accomplishments to plan and machine cistron choice algorithm utilizing CRO, a late proposed optimisation technique.I besides served as a sing module of bioinformatics for biotechnology pupils at Ruia College, Mumbai, which prep ard me for my sphere capable with more duties. It helped me to go more confident and responsible to do others understand bioinformatics in a elusive mode. Teaching microarray informations analysis at University of Pune, Pune and Patkar College, Mumbai besides helped my instruction accomplishments grow. I besides nonionized and conducted a four twenty-four hours workshop on bioinformatics for life scientists at Ruia College. Success of this workshop and incontrovertible r esponse from the participant made me confident about my organisational accomplishments. I have attended big figure of conferences and workshops, have presented my work at national degree conferences therefore have had changeless updates about in the altogether kingdom of biological research. I besides appe ard for BioInformatics depicted object Certification ( BINC ) scrutiny, conducted by Dept. of Biotechnology ( DBT ) , Govt. of India, to prove the efficiency of bioinformatics human resource and certify and congratulate really few of them who deserve to be good bioinformaticians. This running game is of import in manner that it holds three documents, two usual aim and theory inquiries which test your capable cognition and one practical session which examines your aptitude to compose computing machine plans for bioinformatics. I was awarded BINC franchise with All India Rank ( AIR ) 14 and besides a family to prosecute farther research in the field. BINC enfranchisement booste d my assurance about my cognition of bioinformatics. I besides have received grasp from module for good computing machine scheduling and immediately I am involved in teaching of a plan suit for incorporating phyletic analysis package tools to fix an machine-controlled grapevine.Proteins, indispensable biomolecules, are the workhorses of all the cellular maps. Their features and maps are chiefly attributed to their one-third locution. Always amazed by the manner this machinery works I am interested to work on structural bioinformatics of proteins and their interactions with other molecules. My primary research involvements enshroud to be in the maturation of fresh methodological analysiss to understand mechanisms underlying cellular procedures. There are many other research countries in structural biological science which involvement me like molecular mold and molecular kineticss simulation, algorithm development and executions, protein construction development, understanding the protein-protein interactions, anticipation of protein construction all of which are interlinked in a manner or other.I have been introduced to protein construction by high panel of scientists working in this country, and that has elevated my involvements to a great tallness and inspired me to prosecute research in the same. My twelvemonth long research experience in the field of molecular kineticss simulations and molecular moorage, in order to understand the mechanisms of biological procedures and for easing vaccinum development hold given me needed experience. Besides, it introduced me to some of the restrictions and demands of bing methods of protein construction analysis. One of my research involvements is to come up with a newer representation of protein construction for usage in molecular kineticss simulation. soon available methods require truly constitutional calculation power as it involves severe computations on each and every atom of the system. It takes immense ticker of clip on simple machines to run a molecular kineticss simulation. fleck working with assorted simulations, I have observed that inexplicit final result supposed accounts can be good mark to accomplish this end. There is a demand to better these hypothetical accounts in such a manner that they impart come close expressed preparetlement simulations in a better manner. One of the ways in which it might work is deducing forms of interactions from expressed solvent systems and integrating them in inexplicit dissolver metaphysical accounts. Current inexplicit dissolver theoretical accounts include theoretical consequence of solvent molecules but we could better these theoretical accounts by presenting non merely distance dependance, but besides clip dependent solvent interactions. It means that we could present consequence of existent dissolver system at specific clip intervals by adding some solvent molecules and redefine the theoretical account after each interval ther efore propagating the alterations brought approximately by solvent molecules.Another research country which entreaties me most is protein construction anticipation. Looking back in the history of bioinformatics, tremendous attempts have been taken to understand and foretell protein construction, which in bend imparts map to the protein. Our limited success until now after such tremendous attempts illustrates why protein construction anticipation is known as Holy grail of bioinformatics. Though I have had no experience in the country, I find it really interesting country to work upon and use my cognition of protein chemical science, machine acquisition and computing machine scheduling and algorithms to lend to the field.As we look at the of import mileposts in the history of computing machine scientific discipline, we can see distinguishable markers in the development of unreal intelligence. On one manus, biological science is researching expertness from assorted subjects to be util ized in biological research and on the other manus, techniques in computing machine scientific discipline have of all time since widened their pertinence sphere. With this, my profound involvement in computing machines and my passion for biological science has widened my research ends to applications of machine larning techniques in bioinformatics and computational biological science. I have strong experience in utilizing support vector machines and random forest classifiers for work outing simple biological jobs and I worry to travel farther and assist work out much more complex jobs utilizing machine acquisition. Currently I am involved in the development of chemical reaction optimisation ( CRO ) ground cistron choice algorithm. My primary involvement in this country is foretelling accurate ligand adhering sites on a protein construction. Current ligand adhering site anticipation algorithms work on rather generalised rules like geometry ground, energy based etc. I strongly thin k that protein pair sensing for proper ligand binding should be based on more empirical rules and cognition should be brought in from big figure of known protein pits and ligands. categorisation of proteins and ligand into several categories and qualifying each category on certain belongingss seems indispensable to me before using any generalised ligand adhering site anticipation algorithm.Another scene that I would wish to research is protein turn uping and kineticss. more or less of the attempts we have made understand an indispensable procedure of protein turn uping property it to the built-in belongingss of proteins, but many of the experimental consequences have shown that many other factors in the cell contribute to the protein turn uping procedure. Chaperons, microenvironment, different ions besides play a function. I would wish to travel a measuring stick further and seek to roll up all these factors together in a computational paradigm and seek understand turn uping i n better mode. Folding of a protein, which is important measure in finding its maps and interactions, will assist in developing new drug marks and interventions for assorted diseases.These are some of the jobs I would wish to work upon. My long clip calling ends are to set up myself as a research histrion in the field of protein scientific discipline and contribute as per my capablenesss to the society. I besides want to be invariably indulged in learning biological science, as I truly bask learning and share-out my cognition. Besides it makes me more confident with the topic I teach.With my preparation and experiences, and my research and calling ends, I need a suited topographic point where I can use my cognition and experience to develop my thoughts further and set them to people s usage. While seeking for such environment, I was oddly attracted by a really fresh plan in Mathematical, Computational and Systems Biology at University of California, Irvine. Amazing shading of fir st-class research workers from assorted subjects would certainly do a difference. That s why I contumacious to use at UCI. I am peculiarly interested in the work of Ray Luo.